Mxe intein - chitin-binding domain
Webencoded by pTWIN1, the Ssp DnaB intein and the Mxe GyrA intein, cleave the peptide bond at their C- and N-termini, respectively (1-4). The chitin binding domain (CBD) from B. circulans, fused to each intein, facilitates purification of the intein-target protein precursor. Transcription of the intein tags is controlled by the inducible T7 Webviewed in references 3 and 10). The catalytic domain (amino acid residues 50 to 212 [50-212]) contains a conserved D,DX 35 E motif that coordinates the metal ions, Mn 2 or Mg2, that mediate catalysis (14, 19, 42). The catalytic domain also specifies the choice of target DNA sequences for integra-tion in vitro, as shown by the identification ...
Mxe intein - chitin-binding domain
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WebFeb 15, 1993 · Results show that the I domain on the alpha chain of Mac-1 is an important recognition site for all four ligands, and that the NH2-terminal and perhaps divalent cation … WebA polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB (2) and Mxe GyrA inteins (3,4). The presence of the chitin binding …
WebpTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein. View sequence details WebMxe intein - Chitin-binding domain Expression Bacterial Mutation E54K, L372P Promoter T7 promoter Availability Academic Institutions and Nonprofits only Enlarge pET30a-Tn5 Plasmid #127917 Purpose Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation. Depositor Silin Zhong Insert Tn5 Use Tags
WebJan 27, 2004 · Comparison of Mxe GyrA (A) and Sce VMA (B) intein structure. The structures of both inteins have been determined by X-ray crystallography (PDP ... An SH3 domain from murine c-Crk adapter protein with an N-terminal Cys residue was N-terminally fused to an intein with a chitin binding domain. After the expression of this fusion protein, the N ... WebpTYB21 is an E. coli expression N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag.
WebDec 13, 2008 · MXE files are similar to .MEX files, but are only meant to be played back and not edited. The MXE format is commonly used for sharing macros with other users. Open …
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